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目的研究HPVL1壳蛋白与p16检测在诊断宫颈病变及其风险评估的价值。方法对614例因宫颈病变患者行液基薄层细胞学(TCT)和人乳头瘤病毒(HPV)分型检测;对有1项或2项异常的患者进行阴道镜下活检和病理学检查。对HPV检测阳性且TCT正常的205例采用免疫组化法检测HPVL1壳蛋白的表达;对HPV和TCT检测两项异常的86例采用免疫组化法检测p16的表达。其结果并与病理学结果比较。结果 HPVL1壳蛋白的阳性表达率与宫颈病变的病理分级呈负相关,p16蛋白的阳性表达率则与宫颈病变的病理分级呈正相关(P<0.05或P<0.01)。结论 HPVL1壳蛋白及p16可成为诊断宫颈病变及进一步评估风险进展的有效指标。
Objective To study the value of HPVL1 capsid protein and p16 in the diagnosis of cervical lesions and their risk assessment. Methods Sixty-four patients with cervical lesions underwent liquid-based TLCT and HPV typing. Colposcopic biopsy and pathological examination were performed on patients with one or two abnormalities. Totally 205 cases with positive HPV test and normal TCT were used to detect the expression of HPVL1 capsid protein. The expression of p16 was detected by immunohistochemistry in 86 cases of two abnormalities of HPV and TCT. The results are compared with the pathology results. Results The positive rate of HPVL1 capsid protein was negatively correlated with the pathological grade of cervical lesions. The positive rate of p16 protein was positively correlated with the pathological grade of cervical lesions (P <0.05 or P <0.01). Conclusion HPVL1 capsid protein and p16 may be useful indicators for diagnosis of cervical lesions and further assessment of the risk of progression.