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利用一株赭曲霉代谢产物I_(36)N作为脲酶抑制剂研究对土壤脲酶活性的抑制作用,结果表明,I_(36)N对我省主要5种水稻田土壤脲酶活性具有明显的抑制作用。土壤脲酶活性高,I_(36)N抑制作用强,但抑制时间较短。抑制活性随I_(36)N浓度而增强。与化学抑制剂NBPT相比,虽然I_(36)N对土壤脲酶活性的抑制所需用量较大,但抑制时间延长。盆栽试验结果表明,施加I_(36)N后,水层尿素水解速率降低,(NH~+_4+NH_3)—N高峰期比对照推迟2～4天,高峰值也较低。水层pH值变化规律与(NH~+_4+NH_3)—N变化一致。
The inhibitory effect of I_ (36) N, a metabolite of Aspergillus Och as a urease inhibitor, on soil urease activity was studied. The results showed that I_ (36) N significantly inhibited soil urease activity in five main paddy fields in our province. Soil urease activity is high, I_ (36) N inhibition is strong, but the inhibition time is short. Inhibitory activity with I_ (36) N concentration increased. Compared with the chemical inhibitor NBPT, although the amount of I_ (36) N required for the inhibition of soil urease activity was larger, the inhibition time was prolonged. The results of pot experiment showed that after applying I_ (36) N, the rate of urea hydrolysis in aqueous layer decreased, and the peak value of (NH_ + _4 + NH_3) -N was two to four days later than that of control. The change law of pH value of water layer is consistent with that of (NH ~ + _4 + NH_3) -N.