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为了探索板栗疫病的致病机制,将具有致病力的栗疫菌菌株Cfcc82606(Endothia parasitica)的滤液,采用甲醇沉淀和离心得到蛋白和非蛋白类部分,经透析袋以及致病活性检测,该毒素为非蛋白类小分子毒素。对非蛋白类进行不同温度、不同pH值、紫外线照射、甲醇沉淀处理以及透析袋后,测定其致病活性的变化。结果表明:毒素有着较强的热稳定性,经121℃,0.103 MPa处理30min后仍有一定的致病性;毒素的酸碱性敏感,酸性条件下毒性增强,碱性条件下毒性减弱;紫外线的照射对毒素具有削弱作用。经7种不同极性物质萃取后,正丁醇相的致病力最强,该毒素为极性较大的物质。栗疫菌菌株Cfcc82606的非蛋白类毒素对同科属的植物都有着不同程度的致病作用,是一种非寄主类专化性毒素。
In order to explore the pathogenic mechanism of chestnut blight, the pathogenicity of the filtrate of Cfcc82606 (Endothia parasitica) was determined by methanol precipitation and centrifugation to obtain protein and non-protein fractions, which were tested by dialysis bag and pathogenic activity test Toxins are non-protein small molecule toxins. The changes of pathogenic activity of non-protein after different temperature, different pH value, UV irradiation, methanol precipitation treatment and dialysis bag were measured. The results showed that the toxin has strong thermal stability, and still has certain pathogenicity after being treated at 121 ℃ and 0.103 MPa for 30 minutes. The toxin is sensitive to acids and alkalis, the toxicity increases under acidic conditions and the toxicity decreases under alkaline conditions. The ultraviolet The irradiation has a weakened effect on toxins. After the extraction of seven different polar substances, the n-butanol phase has the strongest pathogenicity, and the toxin is a more polar substance. The non-protein toxin of Cfcc82606 is a kind of non-host specialized toxins which has different pathogenicity to plants of the same family.