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目的:探索人参苷皂Rd对人脑胶质瘤的作用。方法:人脑胶质瘤U251细胞系细胞培养,不同浓度的人参皂苷Rd处理观察细胞形态、测定端粒酶活性以及h TERT表达水平。结果:随着GSRd浓度的升高,U251细胞的生长被明显抑制,出现了细胞凋亡和凋亡小体的现象;在经GSRd处理后U251细胞的端粒酶活性,对照组和溶媒组类似,而GSRd药物处理24 h后,细胞端粒酶活性显著降低,其中20μg/L组端粒酶活性降低极显著,P<0.01,40μg/L和80μg/L组端粒酶活性显著降低,P<0.05。GSRd药物处理48 h后,细胞端粒酶在20、40、80μg/L处理后,端粒酶活性降低极显著,P<0.01;20μg/L、40μg/L和80μg/L的GSRd处理细胞后,相比于对照和溶媒组,h TERT基因表达水平显著降低。结论:人参皂苷Rd能够促进人脑胶质瘤U251细胞凋亡,对于临床治疗脑胶质瘤有重要的意义。
Objective: To explore the effect of ginsenoside Rd on human glioma. Methods: Human glioma cell line U251 was cultured in vitro. The cell morphology was observed under different concentrations of ginsenoside Rd. The telomerase activity and the expression of h TERT were measured. Results: With the increase of GSRd concentration, the growth of U251 cells was obviously inhibited and apoptosis and apoptotic bodies appeared. After GSRd treatment, the telomerase activity of U251 cells was similar to that of control and vehicle groups , While telomerase activity was significantly decreased after 24 h GSRd treatment, and the telomerase activity was significantly decreased in 20μg / L group, telomerase activity was significantly decreased at 40μg / L, 40μg / L and 80μg / L, P <0.05. After treated with GSRd for 48 h, the telomerase activity decreased significantly after treated with 20, 40, 80 μg / L of cell telomerase (P <0.01). After treated with 20 mg / L, 40 μg / L and 80 μg / L of GSRd, HTERT gene expression levels were significantly reduced compared to the control and vehicle groups. Conclusion: Ginsenoside Rd can promote the apoptosis of human glioma U251 cells, which is of great significance for the clinical treatment of gliomas.