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目的:检测慢性乙型肝炎(CHB)患者血清IL-21及HBV-DNA、ALT水平,探讨血清IL-21在CHB发生发展中的意义。方法:采用双抗体夹心ELISA法检测63例CHB患者(其中慢性轻度16例,慢性中度25例,慢性重度22例)和20例健康者血清IL-21水平,并同期检测患者HBV-DNA、肝功能等指标。结果:与正常对照组相比,各型CHB患者血清IL-21水平均升高,其中以慢性重度乙型肝炎患者升高最明显(P<0.01);IL-21在ALT异常组明显高于ALT正常组(P<0.01);慢性乙型肝炎HBV-DNA低栽量组与中高载量组之间IL-21水平差异无统计学意义(P>0.05)。结论:IL-21可能参与了CHB抗病毒应答及发病过程,可以在一定程度上反映CHB肝脏内的炎症反应。
Objective: To detect serum levels of IL-21, HBV-DNA and ALT in patients with chronic hepatitis B (CHB) and to explore the significance of serum IL-21 in the development of CHB. Methods: Serum levels of IL-21 in 63 CHB patients (including 16 with mild chronic disease, 25 with chronic moderate disease, 22 with chronic severe disease) and 20 healthy controls were detected by double antibody sandwich ELISA. HBV-DNA , Liver function and other indicators. Results: Compared with the normal control group, the levels of serum IL-21 in all kinds of CHB patients were elevated, among them the patients with chronic severe hepatitis B had the most obvious increase (P <0.01); IL-21 was significantly higher in ALT abnormal group ALT normal group (P <0.01). There was no significant difference in IL-21 level between low-dose HBV-DNA group and medium-high dose group of chronic hepatitis B (P> 0.05). Conclusion: IL-21 may be involved in the antiviral response and pathogenesis of CHB, which can reflect the inflammatory reaction in CHB liver to a certain extent.