【摘 要】
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In this paper,the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method.The res
【机 构】
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Institute of Coal Chemistry,Department of Chemistry
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In this paper,the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method.The result reveals that the adsorption of the protein-calmodulin contains two steps,i.e.,one short quick step followed by a slow one.The complexation of calmodulin with Ca2+ was also first probed using EIS technique,in which the complexation of CaM with Ca2+ could be reflected by the change of apparent membrane capacitance(Capp) clearly.In all above measurements,a redox couple Fe(CN)63-/ Fe(CN)64- was used as probing-pin to reflect all the changes occurring in the above process.Our work suggests that some biological processes of CaM could be studied using EIS method conveniently.
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