论文部分内容阅读
目的 研究中药复方胶囊BRM释介素的抗癌活性及诱导人神经胶质瘤细胞SHG 4 4凋亡。方法 运用MTT法检测BRM对体外人癌细胞株的抑制作用 ;运用小鼠移植瘤模型观察BRM体内抑瘤活性 ;应用电镜、流式细胞仪分析、琼脂糖凝胶电泳观察BRM诱导SHG 4 4。结果 BRM释介素体外对SHG 4 4、MCF 7具有明显的抑制作用 ;体内对小鼠移植性肿瘤表现出较显著的抗癌活性 ;SHG 4 4在BRM水提液作用下发生细胞凋亡。结论 BRM释介素的抗肿瘤活性与诱导细胞凋亡有关
Objective To study the anticancer activity of Chinese herbal compound capsule BRM and induce the apoptosis of SHG 4 4 in human glioma cells. Methods MTT assay was used to detect the inhibitory effect of BRM on human cancer cell lines in vitro. The antitumor activity of BRM in vivo was observed by using mouse xenograft model. The SHM 4 4 induced by BRM was observed by electron microscopy, flow cytometry and agarose gel electrophoresis. RESULTS: BRM angiotensin II had obvious inhibitory effects on SHG 4 4 and MCF 7 in vitro. The in vivo anti-tumor activity of SHG 4 4 showed obvious anti-cancer activity. SHG 4 4 cells developed apoptosis under the BRM water extract. Conclusion The anti-tumor activity of BRM is related to the induction of apoptosis