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目的：建立人血浆中紫杉醇ＨＰＬＣ测定法，为人体内药代动力学研究和药效学评价提供方法学基础。方法：取血浆３００μｌ，以地西泮为内标，采用有机相（叔丁基甲醚）两步提取。常温下氮气吹干，残渣用移动相溶解，进样２０μｌ。色谱条件：ＳｈｉｍＰａｃｋＣＬＣＯＤＳ柱；流动相：甲醇乙腈水（２５∶４０∶３９）；流速为１．２ｍｌ·ｍｉｎ－１，检测波长２２７ｎｍ。结果：本法在０．０３～５．００ｍｇ·Ｌ－１间线性良好，相关系数ｒ＝０．９９９９（ｎ＝７）。高中低浓度的３个质控样品日间内变异性ＲＳＤ都在２．３１％～４．９０％之间，回收率在９７．４％～１０３．３％之间，血浆最低检测浓度为０．００８ｍｇ·Ｌ－１。结论：本方法能简便、准确地测出血浆中微量药物的含量，适于研究需要
OBJECTIVE: To establish a HPLC method for determination of paclitaxel in human plasma, and to provide a methodological basis for pharmacokinetics and pharmacokinetic studies in human. Methods: Take 300μl of plasma and take diazepam as internal standard, and take two steps of organic phase (tert-butyl methyl ether) extraction. Dry at room temperature nitrogen, the residue dissolved with mobile phase, injection 20μl. Chromatographic conditions: Shim PackCLC ODS column; mobile phase: methanol acetonitrile water (25:40:39); flow rate of 1.2ml · min-1, detection wavelength of 227nm. Results: The method was linear in the range of 0.03-5.00 mg · L-1 with a correlation coefficient of 0.9999 (n = 7). The intra-day variability RSDs of three quality control samples with high and low concentrations were between 2.31% and 4.90%, the recoveries were between 97.4% and 103.3%, and the lowest plasma concentrations were 0 .008 mg · L-1. Conclusion: This method can be easily and accurately measure the content of trace drug in plasma, suitable for research needs