以1龄大菱鲆(Scophalmusmaximus)为研究材料,研究养殖水温下大菱鲆消化酶的分布及不同温度和不同pH条件下胃、幽门盲囊、前肠、中肠、后肠中蛋白酶、淀粉酶、脂肪酶的活性。确定饵料中主要营养成分的消化部位和主要消化酶的性质。结果表明,各部位蛋白酶活性的最适温度和pH依次为40℃,2 0;40℃,8 0;60℃,8 0;40℃,8 5;60℃,8 0。18℃养殖水温下,在适宜的pH范围内,各部位蛋白酶活力从高到低依次为幽门盲囊、胃、前肠、中肠、后肠;淀粉酶活性的最适温度和pH依次为40℃,6 5;40℃,8 0;40℃,7 5;50℃,7 0;40℃,8 0;淀粉酶活力从高到低依次为幽门盲囊、前肠、中肠、后肠、胃;脂肪酶活性的最适温度和pH依次为40℃,7 5;40℃,6 5;40℃,7 0;40℃,7 5;40℃,7 5;脂肪酶活力从高到低依次为前肠、中肠、幽门盲囊、后肠、胃。 The first-instar turbot (Scophalmus maximus) was used to study the distribution of digestive enzymes of turbot at turbid water temperature and the activities of protease, starch in the stomach, pyloric caecum, foregut, midgut and hindgut at different temperatures and pH Enzyme, lipase activity. Determine the main nutrient components of the digestive tract digestion and the nature of the digestive enzymes. The results showed that the optimum temperature and pH of protease activity in each part were 40 ℃, 20 ℃, 40 ℃, 80 ℃, 60 ℃, 80 ℃, 80 ℃, 85 ℃, 60 ℃, 8 0.18 ℃ , In the proper pH range, the protease activity of each part from high to low was pyloric sac, stomach, foregut, midgut and hindgut. The optimum temperature and pH of amylase activity were 40 ℃ and 65% 40 ℃, 75 ℃, 70 ℃, 40 ℃, 80 ℃. From high to low, the activity of amylase in the order of pyloric sac, foregut, midgut, hindgut, stomach and lipase The optimum temperature and pH of the activity were as follows: 40 ℃, 75 ℃, 40 ℃, 65 ℃, 40 ℃, 70 ℃, 40 ℃, 75 ℃, 40 ℃, , Midgut, pyloric blind capsule, hindgut, stomach.