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在室内用氰戊菊酯对采集于野外的淡色库蚊进行了选育,获得一个抗性高达6088倍的高抗性品系(RPy),并测定了该品系对常用杀虫剂的交互抗性以及增效剂作用。结果表明,RPy品系对7种拟除虫菊酯的交互抗性范围为6(胺菊酯)~140倍以上(反式-氯菊酯);对P,P′-DDT的交互抗性>388倍;对狄氏剂、乐果、敌百虫和残杀威的交互抗性为1~2倍;对丙体六六六、杀螟硫磷,甲基对硫磷,马拉硫磷、双硫磷和甲萘威的敏感度高于敏感品系。应用各种专一性抑制剂pb、TPP和DMC进行了活体增效作用的研究。结果表明,RPy品系对P,P′DDT的抗性主要与DDT脱氯化氢酶(DDTase)和多功能氧化酶(MFO)有关;对氰戊菊酯的抗性主要与MFO和酯酶有关。
The Culex pipiens pallens collected in the field were screened with fenvalerate indoors to obtain a highly resistant strain (RPy) with a resistance of up to 6088-fold, and the cross resistance of the strain to commonly used insecticides was determined As well as synergist effects. The results showed that the cross resistance of RPy strain to 7 pyrethroids ranged from 6 (tetramethrin) to over 140 times (trans-permethrin), and that of RPE strain was 388 times ; Resistance to dieldrin, dimethoate, trichlorfon and propoxur was 1 to 2 times higher; for hexacarbamide, fenitrothion, methyl parathion, malathion, Phosphorus and carbaryl are more sensitive than sensitive strains. Application of a variety of specific inhibitors pb, TPP and DMC were studied in vivo synergistic effect. The results showed that the resistance of RPy lines to P and P’DDT was mainly related to DDT dehydrogenase (DDTase) and multifunctional oxidase (MFO). The resistance to fenvalerate was mainly related to MFO and esterase.