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目的 从免疫学角度了解重度妊娠高血压综合征 (简称妊高征 )和胎儿生长受限 (fetalgrowth retardation,FGR)时胎盘和羊膜白细胞介素 6 (IL- 6 )的水平。 方法 采用逆转录聚合酶链反应 (RT- PCR)技术检测重度妊高征患者胎盘与羊膜 IL- 6 m RNA的表达。 结果 重度妊高征患者胎盘 IL- 6 m RNA相对含量为 0 .71± 0 .0 7,羊膜 IL- 6 m RNA相对含量为 0 .81± 0 .12 ,均较正常妊娠时为低 (P<0 .0 1,P<0 .0 5 )。合并 FGR组胎盘 IL- 6 m RNA相对含量为 0 .6 8± 0 .0 4,羊膜 IL- 6m RNA相对含量为 0 .76± 0 .0 7,均较不合并 FGR组为低 ,但无统计学差异 (P>0 .0 5 )。 结论 作为免疫因子的 IL- 6可能参与了重度妊高征的免疫损伤过程 ,胎盘或羊膜中 IL- 6含量降低可能是导致FGR发生的原因
Objective To understand the levels of placenta and amniotic membrane interleukin 6 (IL-6) in patients with severe pregnancy-induced hypertension (PIH) and fetal growth retardation (FGR) from an immunological perspective. Methods Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-6 mRNA in placenta and amniotic membrane of patients with severe PIH. Results The relative content of IL-6 mRNA in placenta of severe PIH was 0.71 ± 0. 0 7, and the relative content of IL-6 mRNA in amniotic membrane was 0.81 ± 0.12, which was lower than that of normal pregnancy (P <0 .0 1, P <0. 0 5). The relative content of IL-6 mRNA in the placenta with FGR group was 0.68 ± 0. 04, and the relative content of amniotic IL-6m RNA was 0.76 ± 0.0.7, all of which were lower than those without FGR group, but no Statistical difference (P> 0.05). Conclusions IL-6, as an immunological factor, may be involved in the process of immune injury in severe PIH. The decrease of IL-6 in placenta or amniotic membrane may be the reason of FGR