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目的评价太芪培元颗粒体外对HIV-1病毒的抑制活性。方法 MTT法检测太芪培元颗粒细胞毒性;通过细胞病变法检测太芪培元颗粒对HIV-1急性感染的抑制活性;应用HIV-1 p24抗原ELISA检测太芪培元颗粒对HIV-1急性感染细胞中病毒复制的抑制活性,并计算药物治疗指数。结果太芪培元颗粒细胞毒性实验CC_(50)为(3.761±0.370)mg/m L;合胞体抑制实验EC_(50)为(0.454 5±0.204 6)mg/m L,治疗指数为5.84~12.97;p24抑制实验EC_(50)为(0.56±0.27)mg/m L,治疗指数为5.30~8.74。结论太芪培元颗粒体外抗HIV-1活性较弱。
Objective To evaluate the inhibitory activity of Taiqi Peiyuan granule on HIV-1 virus in vitro. Methods The cytotoxicity of Taiqi Peiyuan Granule was detected by MTT assay. The inhibitory activity of Taiqi Peiyuan Granule on the acute infection of HIV-1 was detected by cytopathic assay. The effect of Taiqi Peiyuan granule on acute HIV-1 infection Inhibitory activity of viral replication in infected cells and calculate drug treatment index. Results CC_ (50) was (3.761 ± 0.370) mg / m L and the EC 50 of syncytia inhibition was (0.454 5 ± 0.204 6) mg / m L and the therapeutic index was 5.84 ~ 12.97; p24 inhibition test EC50 (0.56 ± 0.27) mg / m L, the treatment index was 5.30 ~ 8.74. Conclusion Taiqi peiyuan granules in vitro anti-HIV-1 activity is weak.