为研究一种快速检测食品中副溶血性弧菌的PCR方法,设计一对引物用以检测副溶血性弧菌的热稳定直接溶血素(tdh)基因。扩增产物经电泳,出现与已知阳性对照细菌条带大小一致的条带,即判定该样品为阳性。结果显示PCR方法具有简便、快速、特异、经济等特点,适合国境口岸的特点,可用于食品中副溶血弧菌的常规快速检验。 In order to study a PCR method for rapid detection of Vibrio parahaemolyticus in food, a pair of primers was designed to detect the thermostable direct hemolysin (tdh) gene of Vibrio parahaemolyticus. The amplified product was electrophoresed, and the band with the same size as that of the positive control bacteria was found, that is, the sample was judged as positive. The results showed that the PCR method was simple, rapid, specific, economical and other characteristics suitable for the characteristics of the border crossings can be used for rapid routine testing of Vibrio parahaemolyticus in food.