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目的:研究长梗冬青苷最佳提取工艺条件。方法:采用正交试验设计研究不同浓度的乙醇、乙醇体积、提取时间和提取次数对长梗冬青苷提取率的影响;采用高效液相色谱法测定提取物中长梗冬青苷含量。结果:救必应中长梗冬青苷最佳提取条件为加入6倍量70%乙醇,回流提取3次,每次1 h。结论:本法提取效率高,适合大量提取,可用于工业化生产。
Objective: To study the optimum extraction conditions of longan stems glycosides. Methods: The effects of different concentrations of ethanol, ethanol volume, extraction time and extraction times on the extraction rate of longan stems were studied by orthogonal design. The content of longan stems was determined by high performance liquid chromatography (HPLC). Results: The best extraction conditions for middle and long stems of holly glycosides were as follows: adding 6 times 70% ethanol and extracting 3 times with reflux for 1 hour. Conclusion: This method has high extraction efficiency, suitable for large-scale extraction and can be used for industrial production.