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目的:研究不同蔗糖浓度培养基下右旋糖苷酶Dex与氟化钠Na F联用对变异链球菌(S.Mutans)生物膜形成的影响。方法:体外构建S.Mutans生物膜模型。pH电极检测不同蔗糖浓度下处理因素对生物膜培养基p H值的影响;结晶紫染色法检测不同蔗糖浓度下处理因素对早期生物膜形成的影响;扫描电子显微镜观察处理因素对生物膜形成的抑制作用。结果:不同蔗糖浓度下,Dex组对pH值无明显影响(P>0.05),Na F组pH值均大于对照组,差异有统计学意义(P<0.05),Dex+Na F组的p H值大于对照组(P<0.05);除3%和4%浓度组外,其余各浓度组间差异均有统计学意义(P<0.05)。不同蔗糖浓度下,各处理组生物膜形成量均减少,Dex+Na F各浓度组与对照组相比差异均有统计学意义(P<0.05),Dex和Na F对生物膜的形成具有协同抑制作用。在Dex和Na F联用时,生物膜的形成随着蔗糖浓度增加而逐渐减少。结论:Dex和Na F对S.Mutans生物膜的形成具有抑制作用,两者联用具有协同作用,同时蔗糖浓度能够影响生物膜的形成。
Objective: To study the effects of dextranase Dex and sodium fluoride NaF on the biofilm formation of S. mutans under different sucrose concentrations. Methods: S. Mutans biofilm model was constructed in vitro. pH electrode was used to detect the influence of treatment factors on the pH value of biofilm medium under different sucrose concentrations. The effect of treatment factors on the formation of biofilm at different sucrose concentrations was detected by crystal violet staining. The effects of treatment factors on biofilm formation Inhibition. Results: Dex group showed no significant effect on pH value (P> 0.05), but pH value in NaF group was significantly higher than that of control group (P <0.05), and p value of Dex + NaF group (P <0.05). Except for 3% and 4%, the differences were statistically significant (P <0.05). The biofilm formation of each treatment group was decreased under different sucrose concentrations. There was significant difference between Dex + NaF concentration groups and control group (P <0.05). Dex and NaF were synergistic to biofilm formation Inhibition. Biofilm formation decreases with increasing sucrose concentration when Dex is combined with NaF. Conclusion: Dex and Na F can inhibit the formation of S.Mutans biofilm. The synergistic effect of biosynthesis and biosynthesis of sucrose can be influenced by sucrose concentration.