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全能区域非特异性的胚胎干细胞是研究成体不同脑区控制干细胞分化能力的十分有力的工具。胚胎干细胞源性神经前体细胞移植入成体脑后可分化为功能性神经元,但是未分化的胚胎干细胞在成体脑内各个部位的存活、生长与分化的潜能差异尚不清楚。本文旨在探讨成体脑组织对胚胎干细胞的影响及胚胎干细胞在成体脑内的一系列行为。将少量转绿色荧光蛋白未分化的小鼠胚胎干细胞移植入成体大鼠脑内不同部位,分别于移植5、14和28 d后处死大鼠,进行形态学观察及免疫组化定性,以了解未分化的小鼠胚胎干细胞在大鼠脑内不同区域的存活、生长与分化。结果发现未分化的小鼠胚胎干细胞可逐步整合入受体组织并向nestin阳性神经前体细胞分化。移植细胞及其后裔在海马生长最为旺盛,而在隔区最差(P<0.01);移植细胞分化为神经干细胞的效率也是在海马最高,而在隔区最低(P<0．01)。提示只有部分脑区适合胚胎干细胞及其后裔生存,并提供促进其分化的有益环境。因此,由于位置特异的微环境因子及环境因素的存在,宿主组织特性对决定中枢神经系统疾病的细胞替代疗法策略是相当重要的。
Almighty region Non-specific embryonic stem cells is a very powerful tool to study the ability of adult brain to control stem cell differentiation. Embryonic stem cell-derived neural progenitor cells can differentiate into functional neurons after they are implanted into the adult brain. However, the potential difference between the survival, growth and differentiation of undifferentiated embryonic stem cells in adult brain remains unclear. This article aims to investigate the effect of adult brain tissue on embryonic stem cells and a series of behaviors of embryonic stem cells in adult brain. A small amount of undifferentiated mouse embryonic stem cells transfected with green fluorescent protein were transplanted into different parts of the adult rat brain and rats were sacrificed 5, 14 and 28 days after transplantation respectively. Morphological observation and immunohistochemistry were performed to find out Survival, growth and differentiation of differentiated mouse embryonic stem cells in different regions of rat brain. The results showed that undifferentiated mouse embryonic stem cells can be gradually integrated into the recipient tissue and to nestin positive neural progenitor cells. Transplanted cells and their offspring grew most strongly in the hippocampus, but worst in the septal area (P <0.01). The transplanted cells differentiated into neural stem cells were also the highest in hippocampus and the lowest in the septum (P <0.01). Suggesting that only part of the brain area is suitable for embryonic stem cells and their descendants to survive and provide a beneficial environment for their differentiation. Therefore, due to the presence of site-specific microenvironmental factors and environmental factors, host tissue properties are of considerable importance for cell replacement therapy strategies that determine central nervous system diseases.