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目的:探讨热疗和人抗DR5单克隆抗体(mAb)致肿瘤细胞凋亡的协同作用。方法:使用MTT法检测抗人DR5mAb单独或联合应用顺铂或热疗对肝癌SMMC-7721细胞体外增殖的抑制作用,以流式细胞术检测不同方案对SMMC-7721细胞凋亡率的影响。结果:未热处理细胞增殖抑制率分别为:顺铂组32%;m-DRA-6组22%;顺铂和m-DRA-6组37%。热处理细胞增殖抑制率分别为:顺铂组58.06%;m-DRA-6组14.52%;顺铂和m-DRA-6组91.13%。细胞凋亡的未热处理组:顺铂组13.53%;m-DRA-6组13.66%;顺铂和m-DRA-6组84.24%。热处理组:细胞仅热处理(不加任何药物)对照组9.41%;顺铂组30.27%%;m-DRA-6组57.52%;顺铂和m-DRA-6组91.33%。结论:热疗不仅能明显提高肿瘤细胞凋亡,而且能提高m-DRA-6和化疗药物的致肿瘤细胞凋亡作用。
Objective: To investigate the synergistic effect of hyperthermia and human anti-DR5 monoclonal antibody (mAb) on tumor cell apoptosis. Methods: The inhibitory effects of anti-human DR5 mAb alone or in combination with cisplatin or hyperthermia on the proliferation of SMMC-7721 cells were detected by MTT assay. The effects of different regimens on the apoptosis of SMMC-7721 cells were detected by flow cytometry. Results: The cell proliferation inhibition rates of untreated cells were 32% in cisplatin group, 22% in m-DRA-6 group and 37% in cisplatin and m-DRA-6 group. Heat-treated cell proliferation inhibition rates were: 58.06% in cisplatin group; 14.52% in m-DRA-6 group; 91.13% in cisplatin and m-DRA-6 group. In the untreated group, 13.53% in cisplatin group, 13.66% in m-DRA-6 group and 84.24% in cisplatin group and m-DRA-6 group. The heat-treated group: 9.41% of the cells treated with heat-only (without any drug); 30.27% of the cisplatin group; 57.52% of the m-DRA-6 group; 91.33% of the cisplatin group and the m-DRA-6 group. Conclusion: Hyperthermia can not only significantly improve the apoptosis of tumor cells, but also enhance the apoptosis of tumor cells induced by m-DRA-6 and chemotherapeutics.