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在 Na Cl的胁迫下 ,水稻幼苗根和叶的谷氨酸合酶和谷氨酸脱氢酶的活性随着营养液中的 Na Cl浓度的升高而降低 ;游离 NH+ 4 在叶中积累 ,在根中未见明显变化。与根相比 ,叶对 Na Cl的胁迫作用更为敏感。叶的 NADH- GOGAT和 NADH- GDH活性在 Na Cl胁迫下降低的程度明显大于根。无论是否有 Na Cl存在 ,根的 NADH- GDH活性明显高于叶。 GS/GDH比值分析提示 ,在对照下 ,根中的 NH+ 4 的同化以 GS- GOGAT途径为主 ;而在 Na Cl胁迫下 ,GS- GOGAT途径明显削弱 ,GDH途径在 NH+ 4 的同化中的作用明显加强。无论是否有 Na Cl的存在 ,叶的 NH+ 4 同化途径都是以 GS- GOGAT为主。
Under NaCl stress, glutamate synthase and glutamate dehydrogenase activity in rice seedling roots and leaves decreased with the increase of NaCl concentration in nutrient solution. Free NH + 4 accumulated in leaves, No significant changes in roots. Leaf is more sensitive to NaCl stress than root. The NADH-GOGAT and NADH-GDH activities of leaves decreased significantly under NaCl stress than those of roots. The NADH-GDH activity of roots was significantly higher than that of leaves regardless of the presence of NaCl. GS / GDH ratio analysis indicated that under the control, the assimilation of NH + 4 in root was mainly in GS-GOGAT pathway; while under NaCl stress, GS-GOGAT pathway was significantly weakened, and the role of GDH pathway in NH + 4 assimilation Significantly strengthened. Regardless of the presence of NaCl, the leaf NH + 4 assimilation pathway is mainly GS-GOGAT.