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栎黄掌舟蛾Phalera assimilis是柞树主要叶部害虫之一。本研究以栎黄掌舟蛾幼虫为材料,从幼虫肠道中分离出好氧细菌23株,通过对16S r DNA扩增产物ARDRA分析后,代表性菌株测序结果表明,23株肠道好氧细菌分别属于葡萄球菌属Staphylococcus sp.、短小杆菌属Curtobacterium sp.、赖氨酸芽孢杆菌属Lysinibacillus sp.、肠球菌属Enterococcus sp.和阿特拉津降解菌属Arthrobacter sp.5个属的细菌,其中,以葡萄球菌属及短小杆菌属细菌种类最多。通过筛选培养基从23株菌中筛选出产纤维素酶菌6株。本研究可为深入了解栎黄掌舟蛾肠道菌群结构、寻找产纤维素酶菌及新的微生物资源等奠定了理论基础。
Phalera assimilis is one of the main leaf pests of the oak tree. In this study, 23 species of aerobic bacteria were isolated from the larvae of the larvae of Spodoptera litura. After ARDRA analysis of 16S rDNA amplification products, the sequencing results of representative strains showed that 23 strains of aerobic bacteria Belonging to Staphylococcus sp., Curtobacterium sp., Lysinibacillus sp., Enterococcus sp. And Arthrobacter sp. Arthrobacter sp. , To Staphylococcus aureus and bacteria genus bacteria is the largest. Six strains of cellulase were screened from 23 strains by screening medium. This study can provide a theoretical basis for further understanding of the gut microbiota structure of Quercus acutissima and searching for cellulase producing bacteria and new microbial resources.