论文部分内容阅读
目的 探讨卵圆细胞在肝组织内的起源及其与原发性肝细胞肝癌的关系。方法 清洁型SD大鼠 6 0只 ,随机分为正常组 (2 0只 )和实验组 (40只 ) ,各组于开始制造肝癌模型后的不同时段取肝组织标本进行常规病理和C kit、PCNA免疫组化检测。结果 正常组大鼠肝脏表面光滑 ,组织学形态正常 ,偶见C kit和PCNA阳性细胞 ;实验组于肝脏染毒的第 2周 ,首先于汇管区发现卵圆细胞沿胆管上皮依次排列增生 ,这些卵圆细胞呈C kit和PCNA阳性表达。随着染毒加重 ,卵圆细胞以汇管区为中心向肝小叶穿插生长。肝癌形成时 ,癌结节内外均见有卵圆细胞聚集 ,此期C kit阳性细胞仍以汇管区为主 ,而PCNA阳性细胞遍布癌结节内外。结论 卵圆细胞起源于汇管区的胆管上皮 ;卵圆细胞与肝癌的发生密切相关。
Objective To investigate the origin of oval cells in liver tissue and its relationship with primary hepatocellular carcinoma. Methods Sixty clean SD rats were randomly divided into normal control group (n = 20) and experimental group (n = 40). The liver tissue samples were taken from the liver cancer models at different time points after the start of the model of hepatocellular carcinoma for routine pathology and C kit, PCNA immunohistochemistry. Results In the normal control group, the liver surface was smooth and histological morphology was normal. C kit and PCNA positive cells were occasionally seen. In the experimental group, the first 2 weeks after the liver exposure, the oval cells were arranged in order along the biliary epithelium in the portal area. Oval cells were C kit and PCNA positive expression. With increased exposure, oval cells to the portal area as the center of hepatic lobule interspersed growth. In the formation of hepatocellular carcinoma, oval cells were found both inside and outside of cancerous nodules. C kit positive cells were still mainly located in portal area during this period, while PCNA positive cells were found throughout the nodules. Conclusion Oval cells originate from the bile duct epithelium in the portal area. Oval cells are closely related to the occurrence of hepatocellular carcinoma.