论文部分内容阅读
目的:观察结直肠三硝基苯磺酸(TNBS)致炎条件下,改变大鼠体内雌激素和孕激素水平对背根神经节(DRG)中P2X3和P2X2mRNA表达的影响。方法:SD大鼠分为4组:假手术组、去卵巢组、去卵巢加雌激素替代组、去卵巢加雌孕激素替代组,每组手术完成后饲养5周,再行TNBS致炎,5～7d后用于实验。实时定量RT-PCR法检测各组P2X3和P2X2mRNA的表达。结果:去卵巢组大鼠DRG中P2X3mRNA表达明显低于假手术组,差异有统计学意义(1.08±0.35vs1.66±0.66,P<0.05,n=4),雌激素替代组有明显上升(2.06±0.58),明显高于去卵巢组(P<0.01,n=5),而雌孕激素同时替代组明显低于单独添加雌激素组(2.06±0.58vs0.87±0.28,P<0.01,n=5)。各组大鼠DRG中P2X2mRNA表达无统计学差异。结论:结直肠TNBS致炎条件下,雌激素可促进大鼠DRG中P2X3mRNA表达,但对P2X2mRNA无明显影响;孕激素的作用可能与雌激素正好相反。炎性条件下,雌孕激素可能通过影响P2X3受体的表达发挥调节炎性痛作用。
OBJECTIVE: To observe the effects of estrogen and progestin levels in rats on the expression of P2X3 and P2X2 mRNA in the DRG of rats under the inflammation of trinitrobenzene sulfonic acid (TNBS). Methods: The SD rats were divided into 4 groups: sham-operation group, ovariectomized group, ovariectomized plus estrogen replacement group and ovariectomized plus estrogen-progesterone replacement group. Each group was kept for 5 weeks after operation, 5 ~ 7d after the experiment. Real-time quantitative RT-PCR method was used to detect the expression of P2X3 and P2X2 mRNA in each group. Results: The expression of P2X3 mRNA in DRG of ovariectomized rats was significantly lower than that of sham operated rats (1.08 ± 0.35 vs 1.66 ± 0.66, P <0.05, n = 4), estrogen replacement group was significantly increased 2.06 ± 0.58), which was significantly higher than that of the ovariectomized group (P <0.01, n = 5), while that of estrogen and progesterone replacement group was significantly lower than that of estrogen and estrogen alone groups (2.06 ± 0.58 vs 0.87 ± 0.28, P <0.01, n = 5). There was no significant difference in P2X2 mRNA expression in DRG of each group. CONCLUSION: Estrogen can promote P2X3 mRNA expression in DRG of rats under the condition of colitis induced by TNBS, but has no obvious effect on P2X2 mRNA. The effect of progesterone may be opposite to that of estrogen. In inflammatory conditions, estrogen and progesterone may play a role in regulating inflammatory pain by affecting the expression of P2X3 receptor.