从细胞超微结构水平动态观察了在不同浓度和不同作用时间下,半合成性中药高三尖杉酯碱对体外培养的人眼结膜成纤维细胞的作用及其机理。初步结果表明,该药引起细胞损伤的主要特点是核膜内陷,染色质凝聚和边集以及胞浆的广泛空泡变性。此外,该药尚能抑制细胞分泌胶原纤维和合成细胞微丝的功能,故其为防治眼内纤维增生性病变的有效药物。 The effect of semi-synthetic Chinese homoharringtonine on cultured human eye conjunctival fibroblasts at different concentrations and different time was observed dynamically from the ultrastructure of cells. Preliminary results show that the drug-induced cell damage is characterized by nuclear invagination, chromatin condensation and edge set, and extensive vacuolar degeneration of the cytoplasm. In addition, the drug is still able to inhibit the secretion of collagen fibers and synthetic cells actin filaments, so it is an effective drug for the prevention and treatment of intraocular fibrosis.