【摘 要】
：
In this paper, the distribution and expression of diapause hormone (DH) at mRNA and protein levels in the central nervous system of Bombyx mori embryo and larva
【机 构】
：
Department of Molecular and Cell Biology,Institute of Sericultural Research
论文部分内容阅读
In this paper, the distribution and expression of diapause hormone (DH) at mRNA and protein levels in the central nervous system of Bombyx mori embryo and larva were studied using whole-mount in situ hybridization and immunocytochemistry. Whole-mount immunocytochemistry revealed that the distribution of the DH-like immunoreactivity was throughout the central nervous system including the brain, suboesophageal ganglion (SG) and thoracic ganglia (TG); and that the corpus cardiacum and terminal abdominal ganglion may be the site for DH release due to the presence of strong immunoreactivity. In situ hybridization with the probe labeled by digoxigenin shows that the Bom-DH mRNA was also localized in the mandibular, maxillary, labial cell clusters. In addition, a pair of lateral neurons in the SG and a pair of ventral midline neurons in each TG expressing the Bom-DH transcript were also identified. These results were consistent with the localization of Bom-DH mRNA in larva by in situ hybridization and the distribution of the gene by RT-PCR, which is some different from the results reported previously.
其他文献
Occurrence and development of lymphocystis cells in flounder Paralichthys olivaceus, and some histochemical features are studied using light and electron micros
Objective To study the effect of gene radiotherapy combining injection of recombinant plasmid pNEgr-mIL-12 with local X-irradiation on cancer growth and to eluc
Methods and techniques for the identification, monitoring and management of natural hazards in high mountain areas are enumerated and described. A case study fr
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7
Chemokines are a superfamily of small proteins that bind to G protein-coupled receptors on target cells and were originally discovered as mediators of direction
With biological and economic importance,threshold traits are discrete in phenotype but have the same polygenic genetic basis as quantitative traits. The traditi
利用热天平和小型煤粉燃烧实验台对无烟煤、贫煤及其三种配比混煤的燃烧特性、不同配风下NOx的生成规律和燃尽特性进行了实验研究。通过对试验数据的整理和分析,认为无烟煤与
Tourism development surely has social, economic and environmental impacts on tourist destinations.The study on resorts residents attitudes can help understand t
Objective To investigate the anti-HIV effects of ampelopsin and its interaction with HIV-1 coreceptor CXCR4. Methods Through anti-virus experiments in vitro, th
After male sterile rice plants are inoculated with secondary sporidia of Neovossia horrida in the field, the process of infection is comparatively studied in fo