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在分子水平研究四川省芋资源的遗传多样性和亲缘关系,为芋种质资源的分类、保护和有效利用遗传资源以及新品种选育提供依据。本研究利用SRAP分子标记技术,使用28对SRAP引物组合对65份四川省不同地区芋种质资源材料进行遗传多样性分析,采用NT-SYS 2.1统计软件对数据进行分析,建立树状聚类图。扩增出并检测到341条条带,平均每个引物组合扩增检测出12.18条带,多态性带251条,多态率73.6%。UPGMA树形图表明,所用的SRAP引物组合可以将65份材料分成5类,分别与这些材料在园艺分类学上按母芋和子芋的生长习性分类基本相符,与以芋叶心色斑颜色、叶柄中下部颜色、母芋芽色及母芋肉色4种形态性状组合描述具有相关性。研究表明,从四川省不同地区、不同生态环境下收集的不同类型芋种质资源间存在着较丰富的遗传多样性,SRAP分析聚类结果与主要形态学性状分类基本一致,可以解释芋栽培种的进化关系。
At the molecular level, the genetic diversity and genetic relationship of taro resources in Sichuan Province are studied. It provides the basis for the classification of taro germplasm resources, the protection and effective utilization of genetic resources and the breeding of new varieties. In this study, SRAP molecular marker technology was used to analyze the genetic diversity of 65 taro germplasm resources in 65 different regions of Sichuan Province by using 28 pairs of SRAP primer combinations. NT-SYS 2.1 statistical software was used to analyze the data to establish a tree-like clustering map . A total of 341 bands were amplified and detected. On average, 12.18 bands were detected and amplified with each primer combination. There were 251 polymorphic bands with a polymorphism rate of 73.6%. UPGMA tree map shows that the SRAP primer combinations used can be divided into 65 categories of materials divided into five categories, respectively, with the classification of these materials in the horticultural taxonomy according to the growth habits of the parent taro and sub-taro basically consistent with the heart taro leaf color, Petiole in the lower part of the color, mother taro bud and mother taro color combinations of four kinds of morphological description has relevance. The results showed that there existed abundant genetic diversity among the different types of taro germplasm collected from different regions and different ecological environments in Sichuan Province. The clustering results of SRAP were basically the same as the classification of main morphological traits, The evolutionary relationship.