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实验用抗ＦＯＳ蛋白和酪氨酸羟化酶（ＴＨ）的免疫组织化学方法，观察大鼠暴露于８Ｈｚ，１２０ｄＢ的次声２ｈ后，延髓ＦＯＳ的表达情况及其与儿茶酚胺能神经元的关系。结果显示，在延髓内出现大量ＦＯＳ样免疫反应阳性神经元（ＦＬＮ），主要分布在延髓中尾段的孤束核、腹外侧区以及两者之间的网状结构内；在抗ＦＯＳ和抗ＴＨ的双重免疫染色切片上，见到大约５０％的ＴＨ阳性神经元的胞核同时呈ＦＯＳ样免疫反应阳性，主要分布在腹外侧网状核和孤束核内侧亚核，说明在延髓内脏带有半数儿茶酚胺能神经元参与对次声刺激的反应。
Experimental anti-FOS protein and tyrosine hydroxylase (TH) immunohistochemical method to observe the rats exposed to 8Hz, 120dB infrasound for 2h, the medulla oblongata FOS expression and its relationship with catecholaminergic neurons. The results showed that a large number of FOS-like immunoreactive neurons (FLN) were found in the medulla oblongata, which mainly distributed in the solitary nucleus, ventral part of the medulla oblongata and the reticular formation between the two. In the anti-FOS and anti-TH Of the double immunostaining sections, about 50% of the TH-positive neurons showed positive FOS-like immunoreactivity at the same time, mainly in the ventral reticular nucleus and the medial nucleus of solitary nucleus, indicating that in the medullary viscera with Half of catecholaminergic neurons are involved in the response to infrasound stimulation.