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目的了解南平地区2006-2007年乙型肝炎病毒的分子流行病学方法。方法对8县区综合医院210份乙型肝炎感染者,采用荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA含量,ELISA方法检测血清标志物(HBV-M),PCR-RFLP方法对基因分型。结果 HBsAg+/HBcAb+/HBeAg+组HBV-DNA的阳性率为94.9%,HBV-DNA含量也最高。HBsAg阳性组与HBsAg阴性组的HBV-DNA含量差异有统计学意义,HBeAg阳性组与阴性组的HBV-DNA含量差异有统计学意义。结论 HBV-DNA含量与HBsAg、HBeAg的存在明显关联。HBV-DNA含量可真实反应HBV的感染和复制情况,对临床诊断、药物治疗及疗效观察有重要的指导意义。
Objective To understand the molecular epidemiology of hepatitis B virus in Nanping area from 2006 to 2007. Methods 210 patients with hepatitis B in eight county general hospitals were enrolled in this study. The levels of HBV DNA were detected by FQ-PCR, serum HBV-M by ELISA and PCR-RFLP Genotyping. Results The positive rate of HBV-DNA in HBsAg + / HBcAb + / HBeAg + group was 94.9% and HBV-DNA content was the highest. The HBV-DNA content of HBsAg-positive group and HBsAg-negative group had significant difference, and the difference of HBV-DNA content between HBeAg-positive group and negative group was statistically significant. Conclusion HBV-DNA content is significantly associated with the presence of HBsAg and HBeAg. HBV-DNA content can truly reflect the infection and replication of HBV, which has important guiding significance for clinical diagnosis, drug treatment and curative effect observation.