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目的:研究灰色链霉菌对芦丁的生物转化及其产物对超氧阴离子(O2·-)的清除能力变化。方法:用标准马铃薯培养基培养的灰色链霉菌中加入底物后继续培养4天,发酵液经萃取后采用硅胶柱层析分离纯化,产物的结构根据光谱数据予以鉴定。通过化学发光法测定转化产物对邻苯三酚-鲁米诺-碳酸缓冲液(pH10.2)体系产生的O2·-的清除能力。结果:分离鉴定了6个代谢产物,分别是槲皮素3OβD葡萄糖苷、槲皮素、山柰酚3OβD芸香糖苷、异鼠李素、异鼠李素3O葡萄糖苷和山柰酚,其中4个化合物的抗氧化活性比底物芦丁强。结论:此转化过程涉及到3类反应即糖苷水解、甲基化和去羟基化反应,其中苷类的去羟基化反应为首次发现。本研究表明微生物转化是增加天然化合物多样性的有效方法。
Objective: To study the biotransformation of rutin by Streptomyces griseus and its scavenging ability on superoxide anion (O2 · -). Methods: Streptomyces griseus was cultured in standard potato culture medium for 4 days. The fermentation broth was extracted and purified by silica gel column chromatography. The structure of the product was identified by spectral data. The chemiluminescence method was used to determine the scavenging ability of the conversion products to O2 · - produced by the pyrogallol-luminol-carbonate buffer (pH10.2) system. Results: Six metabolites were isolated and identified as quercetin 3OβD glucoside, quercetin, kaempferol 3OβD rutinoside, isorhamnetin, isorhamnetin 3O glucoside and kaempferol, of which 4 The antioxidant activity of the compound is stronger than the substrate rutin. CONCLUSION: This transformation involved three types of glycoside hydrolysis, methylation and dehydroxylation reactions, of which the dehydroxylation of glycosides was the first discovery. This study shows that microbial transformation is an effective way to increase the diversity of natural compounds.