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目的应用商品化多重PCR试剂盒来检测致泻性大肠埃希菌,以建立更快速、高效的致泻性大肠埃希菌监测工作方法。方法应用某市场商品化致泻性大肠埃希菌多重PCR试剂盒来初筛检测福建省的腹泻病分离的菌株,用本实验室前期已建立的致泻性大肠埃希菌不同的PCR方法进行串联试验对初筛结果加以验证。结果验证试验从2015年福建省2个监测点分离的经生化反应初步鉴定为大肠埃希菌的菌株中,致泻性大肠埃希菌鉴定率为7.00%(21/300),阳性菌株为21株,均成功获得了分纯单克隆菌株。结论商品化致泻性大肠埃希菌多重PCR试剂盒对于提高效率、减少繁重的工作量有帮助。
Objective To detect diarrheal Escherichia coli using commercial multiplex PCR kit to establish a more rapid and efficient method of monitoring diarrheogenic Escherichia coli. Methods A commercial diarrhea-resistant Escherichia coli multiplex PCR kit was used to screen the strains isolated from diarrheal diseases in Fujian Province. The strains were isolated from the diarrhea-resistant Escherichia coli previously established in our laboratory by different PCR methods Serial test to verify the results of the preliminary screening. Results Validation test Among the isolates of Escherichia coli biochemically isolated from 2 monitoring sites in Fujian Province in 2015, the identification rate of diarrheal Escherichia coli was 7.00% (21/300) and the positive strain was 21 Strain, were successfully obtained points of pure monoclonal strains. Conclusion The commercial diarrhea-prone Escherichia coli multiplex PCR kits help to improve efficiency and reduce heavy workload.