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对日本沼虾肝胰腺细胞进行培养,采用M199(GIBCOBRL)细胞培养基加质量分数为10%的胎牛血清,添加的补充成分有:NaCl,NaHCO3,MgCl2,CaCl2,葡萄糖,青霉素和链霉素.同时对pH和渗透压两因子进行了研究,结果表明pH7.0～7.2、渗透压为600μmol/g时,细胞长势最好.以此作为培养条件,在培养基中加入促细胞生长因子的亚油酸,发现其促生长作用明显,其中亚油酸浓度为16×10-5mol/L时,促进作用最好.日本沼虾肝胰腺细胞共继代2次,存活时间约45d.
Macrobrachium nipponense hepatopancreas cells were cultured using M199 (GIBCOBRL) cell culture medium supplemented with 10% fetal calf serum supplemented with NaCl, NaHCO3, MgCl2, CaCl2, glucose, penicillin and streptomycin At the same time, the two factors of pH and osmotic pressure were studied, and the results showed that when the pH value was between 7.0 and 7.2 and the osmotic pressure was 600μmol / g, the cell growth was the best.As the culture condition, the growth factors Linoleic acid, and found that its promotion of growth was obvious, of which linoleic acid concentration of 16 × 10-5mol / L, the promotion of the best.Monorhabdus japonicus hepatopancreas cells in two generations, the survival time of about 45d.