探索了GC/FID定量分析DNA修饰碱基 5 甲基胞嘧啶和 8 羟基鸟嘌呤的实验条件 ,用GC/MS鉴定各有关成分。结果表明 ,DNA水解物中不同成分可被成功地衍生和分离 ;5 甲基胞嘧啶和 8 羟基鸟嘌呤的相对摩尔反应因子分别为 3 0和 1 3;灵敏度分别为 5 50× 1 0 9mV·s/ g和 7 59× 1 0 1 0 mV·s/ g ;检测限可分别达 36 4pg/s和 1 5 8pg/s ;整个分析流程的相对标准偏差小于 2 0 %。 The GC / FID quantitative analysis of DNA modified base 5 methyl cytosine and 8 hydroxyl guanine experimental conditions were identified by GC / MS of the relevant components. The results showed that the different components of DNA hydrolysates could be successfully derived and isolated. The relative molar response factors of 5-methylcytosine and 8-hydroxyguanine were 30 and 1 3, respectively. The sensitivities were 5 50 × 109 mV · s / g and 7 59 × 10 11 mV · s / g. The detection limits were 36 4 pg / s and 15 8 pg / s, respectively. The relative standard deviation of the whole analysis process was less than 20%.