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用重离子7Li和12C以及60Co的γ射线分别辐照玉米种子胚,研究重离子束辐照玉米种子M1代的生物学效应.结果表明:60Co的γ射线使种子的发芽势和发芽率降低,重离子7Li和12C对发芽势和发芽率的抑制作用不明显;重离子辐照后,单株之间光合速率差异增大;M1代植株在雄性育性、叶片颜色及株高等方面发生了多种变化,并产生了一株双胚苗.应用随机多态性DNA分析技术,发现双胚苗的大、小苗之间及其与亲本自交系478在随机多态性DNA指纹上存在的差异,从分子水平上初步证实了双胚苗为自交系478的变异类型.
The biological effects of heavy ion irradiation on maize seed M1 were studied by irradiating corn seed embryos with heavy ions 7Li and 12C and 60Co γ rays respectively.The results showed that 60Co gamma rays reduced the seed germination energy and germination rate, The inhibitory effects of heavy ions 7Li and 12C on germination potential and germination rate were not obvious. After heavy ion irradiation, the difference of photosynthetic rate between single plants increased. The M1 plants had more fertility in male fertility, leaf color and plant height And produced a double embryo seedling.Using random amplified polymorphic DNA analysis technology, we found that there was a difference in DNA fingerprinting between large and small seedlings and their parents inbred line 478 with random polymorphism , Initially demonstrated from the molecular level the inbred line 478 double-embryo seedling mutant types.