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昆虫病原细菌的研究和应用日益受到重视,实验室也需要经常配制细菌悬浮液。通常菌悬液浓度的测定采用显微计数,此法效率很低且因测定者人为影响使各次测定结果有较大的相对误差。现今的实验室几乎都配备光电比色计和分光光度计,用此类仪器测定菌悬液浓度,简便、快速而准确。 原理:一定浓度的细菌悬浮液有一定的消光度,浓度与消光度呈正比,且在一定的
The research and application of entomopathogenic bacteria are paid more and more attention. The laboratory also needs to prepare the bacterial suspension frequently. Usually the determination of the concentration of bacterial suspension using microscopic counting, this method is very low and due to the determination of human influence so that the determination of the results of a larger relative error. Nearly all of today’s laboratories are equipped with a photoelectric colorimeter and spectrophotometer, with such instruments to determine the concentration of bacterial suspension, simple, fast and accurate. Principle: a certain concentration of bacterial suspension has a certain degree of extinction, concentration and extinction was proportional to, and in a certain