于2009年的7-10月间在辽宁省的桓仁,吉林省的集安、靖宇、抚松等药材产区采集人参及人参根际土壤样品45份。通过真菌分离和培养,共获得真菌菌株105株,经形态学鉴定分属于15属48种。通过活性筛选,得到具有转化人参总皂苷活性的菌株25株,其中菌株SR87和SR105对人参皂苷Rb1具有专一转化活性。通过TLC和HPLC检测,其转化产物为人参皂苷Rd。经形态学鉴定,确定阳性菌株SR87为莫勒接霉Zygorhynchus moelleri,SR105为灰绿犁头霉Absidia glauca。这两株真菌均有较高的转化潜力,可以应用于制备人参皂苷Rd。 45 samples of ginseng and ginseng rhizosphere soil were collected from July to October 2009 in Huanren of Liaoning Province, Ji’an, Jingyu and Fusong of Jilin Province. By fungal isolation and culture, a total of 105 strains of fungi were obtained, which were classified into 15 genera and 48 species by morphological identification. Twenty-five isolates were obtained through active screening, of which strain SR87 and SR105 had specific conversion activity to ginsenoside Rb1. Its conversion product was ginsenoside Rd by TLC and HPLC. Morphological identification showed that the positive strain SR87 was Zygorhynchus moelleri and SR105 was Absidia glauca. Both strains have higher conversion potential and can be applied to the preparation of ginsenoside Rd.