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目的探讨多聚ADP核糖聚合酶(PARP)抑制剂3-氨基苯甲酰(3-AB)对实验性糖尿病(DM)大鼠血脑屏障(BBB)的影响。方法实验动物分为正常对照组(control组),糖尿病组(DM组),糖尿病加3-AB组(DM加3-AB组),3个月后检测各组大鼠BBB通透性,BBB紧密连接蛋白ZO-1和occludin的表达;Western blot法检测各组大鼠PARP1,p-ERK1/2和ERK1/2的表达。结果和control组相比,DM组体质量显著下降(P<0.01),血糖明显升高(P<0.01),3-AB对DM大鼠的体质量和血糖没有显著影响。DM组大鼠BBB通透性显著增加(P<0.01),ZO-1和occludin的表达显著降低(P<0.01),PARP1的表达明显增加(P<0.01),p-ERK1/2/ERK1/2的表达显著增加(P<0.01)。上述作用可被3-AB抑制。结论应用3-AB可通过增加ZO-1和occludin的表达,降低DM大鼠BBB通透性,保护DM大鼠的BBB。其保护作用可能与ERK1/2通路有关。
Objective To investigate the effect of poly (ADP-ribose polymerase) 3-aminobenzoyl (3-AB) on blood-brain barrier (BBB) in experimental diabetic rats. Methods The experimental animals were divided into control group, diabetic group (DM group) and diabetes plus 3-AB group (DM plus 3-AB group). After 3 months, the BBB permeability, BBB The expression of tight junction proteins ZO-1 and occludin were detected by Western blot. The expressions of PARP1, p-ERK1 / 2 and ERK1 / 2 were detected by Western blot. Results Compared with the control group, the DM group had significantly decreased body weight (P <0.01) and blood glucose (P <0.01). The 3-AB had no significant effect on body weight and blood glucose in DM rats. The BBB permeability in DM group was significantly increased (P <0.01), the expression of ZO-1 and occludin was significantly decreased (P <0.01), the expression of PARP1 was significantly increased (P <0.01) 2 expression was significantly increased (P <0.01). This effect can be inhibited by 3-AB. Conclusion The application of 3-AB can reduce the BBB permeability of DM rats and protect the BBB of DM rats by increasing the expression of ZO-1 and occludin. Its protective effect may be related to the ERK1 / 2 pathway.