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目的研究转录因子激活蛋白-2α(Transcription factor activator protein-2α,AP-2α)对滋养细胞Be Wo凋亡、侵袭等生物学功能的影响,探讨在子痫前期(preeclampsia,PE)发生发展中的作用。方法将已构建的p IRES2-EGFP/AP-2α真核表达载体,采用瞬时转染的方法转入滋养细胞系Be Wo细胞中,应用real timePCR及Western blot方法,检测各组Be Wo细胞中AP-2αm RNA及蛋白表达;采用流式细胞仪检测细胞凋亡,Transwell法检测转染后各组细胞侵袭力。结果 AP-2α转染Be Wo细胞后AP-2αm RNA和蛋白表达量较对照组显著增加,其差异有统计学意义(P<0.05)。流式细胞仪检测细胞凋亡结果显示:与对照组比较,AP-2α转染组细胞凋亡数量明显增多,差异有统计学意义(P<0.05);Transwell检测细胞侵袭能力实验结果显示:与对照组比较,AP-2α转染组细胞侵袭的数量显著减少,差异有统计学意义(P<0.05)。结论证明AP-2α促进滋养细胞凋亡并降低其侵袭力,造成滋养细胞层浅表植入,从而为子痫前期致病机理研究提供了理论依据。
Objective To investigate the effects of Transcription factor activator protein-2α (AP-2α) on the biological functions of apoptosis and invasion of Be Wo in gestational trophoblasts and to explore the role of AP-2α in the development and progression of preeclampsia effect. Methods The eukaryotic expression vector pIRES2-EGFP / AP-2α was transiently transfected into Be Wo cells. Real time PCR and Western blot were used to detect AP -2αm RNA and protein expression were detected by flow cytometry. Transwell method was used to detect the invasiveness of cells in each group. Results The AP-2α mRNA and protein expression of AP-2α transfected Be Wo cells were significantly increased compared with the control group (P <0.05). Flow cytometry showed that the number of apoptotic cells in AP-2α transfected cells was significantly higher than that in control cells (P <0.05). The results of Transwell assay of cell invasion showed that: Compared with the control group, the number of cell invasion in AP-2α transfected group was significantly decreased (P <0.05). Conclusion The results suggest that AP-2α can promote the apoptosis of trophoblasts and reduce the invasiveness of trophoblast cells, resulting in the superficial implanted of trophoblastic layer, which provides a theoretical basis for the study of pathogenesis of preeclampsia.