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用培养的兔腹腔巨噬细胞条件培养基作为趋化因子的来源，用改良的Ｂｏｙｄｅｎ小室微孔滤膜法进行单核细胞趋化试验，观察了兔腹腔巨噬细胞条件培养基对单核细胞的趋化作用和抗单核细胞趋化蛋白－１（ｍｏｎｏｃｙｔｅｃｈｅｍｏａｔｔｒａｃｔａｎｔｐｒｏｔｅｉｎ－１，ＭＣＰ－１）抗体对单核细胞迁移的影响。同时用Ｎｏｒｔｈｅｒｎｂｌｏｔ分析方法检测了ＭＣＰ－１ｍＲＮＡ在该巨噬细胞的表达。结果显示，该条件培养基对单核细胞有明显的趋化作用，并被抗ＭＣＰ－１抗体所抑制。同时该巨噬细胞也能表达ＭＣＰ－１ｍＲＮＡ。这提示，巨噬细胞能分泌ＭＣＰ－１，并招引单核细胞迁入内皮下间隙，从而在动脉粥样硬化的发病过程中发挥重要的作用。
Cultured rabbit peritoneal macrophages conditioned medium as a source of chemokines, modified Boyden chamber microporous membrane monocyte chemotaxis assay was observed rabbit peritoneal macrophages conditioned medium monocytes And chemotactic effect of monocyte chemoattractant protein-1 (MCP-1) on monocyte migration. At the same time, the expression of MCP-1 mRNA in macrophages was detected by Northern blot analysis. The results showed that the conditioned medium had obvious chemotactic effect on monocytes and was inhibited by anti-MCP-1 antibody. At the same time the macrophages can also express MCP-1 mRNA. This suggests that macrophages secrete MCP-1, and attract mononuclear cells to migrate into the subepithelial space, which plays an important role in the pathogenesis of atherosclerosis.