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目的：探讨西洋参茎叶二醇三醇组皂甙（PQTS）对大鼠离体工作心脏血流动力学的影响及其作用机理。方法：制备大鼠离休工作心脏，观察三种剂量（30，100和200mg／L）PQTS的作用。提高细胞外液Ca2+浓度（0．56g／L）观察Ca2+对100mg／LPQTS作用的影响。另外向灌流液中加入异丙肾上腺素（Isop1×10-9g／L），观察IsoP对100mg／l。PQTS作用的影响。结果：PQTS具有负性肌力和负性频率作用，并呈现明显的量效关系。100mg／LPQTS可以拮抗因Ca2+浓度增加所致的正性肌力作用，但不能对抗Isop所致的正性肌力作用。结论：PQTS具有负性肌力和负性频率作用。其机理可能是因抑制Ca2+内流所致，而与β-受体无关。
Objective: To investigate the effect and mechanism of PQTS on hemodynamics of isolated working heart in rats. Methods: The working heart of rats was retired, and the effects of three doses of PQTS (30, 100 and 200 mg / L) were observed. Increase the concentration of extracellular Ca2 + (0.56g / L) to observe the effect of Ca2 + on the role of 100mg / LPQTS. In addition, isoprenaline (Isop1 × 10-9g / L) was added to the perfusate to observe the IsoP to 100mg / l. Effect of PQTS. Results: PQTS had negative inotropic and negative frequency effects and showed a significant dose-response relationship. 100mg / LPQTS can antagonize the positive inotropic effect caused by the increase of Ca2 + concentration, but not the positive inotropic effect caused by Isop. Conclusion: PQTS has negative inotropic and negative frequency effects. The mechanism may be due to inhibition of Ca2 + influx, but not with the β-receptor.