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本文探讨了提高体外大量扩增调节性T细胞(Treg)及其免疫抑制功能的方法。免疫磁珠分选的人的CD4+CD25+T细胞,用抗-CD3/CD28包被的免疫磁珠刺激,加入外源IL-2和免疫抑制剂雷帕霉素(Rapa),分为对照组(未加TGF-β1)和实验组(加TGF-β1),培养12d。结果显示,实验组表达HLA-DR与Foxp3的细胞百分比高于对照组,实验组的平均荧光强度也高于对照组;实验组的免疫抑制功能强于对照组;实验组分泌的炎症细胞因子显著减少。因此,人CD4+CD25+T细胞在抗CD3/CD28包被的免疫磁珠体外刺激扩增过程中加入TGF-β1可以上调CD4+CD25+T细胞Foxp3和HLA-DR表达量,提高其纯度和免疫抑制功能。
This article explored ways to increase large numbers of expanded regulatory T cells (Tregs) and their immunosuppressive properties in vitro. Human CD4 + CD25 + T cells isolated by immunomagnetic beads were stimulated with anti-CD3 / CD28 coated magnetic beads and added with exogenous IL-2 and Rapamycin (Rapa) Group (without TGF-β1) and experimental group (plus TGF-β1), cultured for 12 days. The results showed that the percentage of cells expressing HLA-DR and Foxp3 in the experimental group was higher than that in the control group, and the average fluorescence intensity in the experimental group was also higher than that in the control group. The immunosuppressive function of the experimental group was stronger than that of the control group. The inflammatory cytokines secreted by the experimental group were significantly cut back. Therefore, when human CD4 + CD25 + T cells were stimulated with anti-CD3 / CD28 coated magnetic beads in vitro, TGF-β1 could up-regulate Foxp3 and HLA-DR expression in CD4 + CD25 + T cells and increase their purity Immunosuppressive function.