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气相色谱方法在生化医学和临床方面的广泛应用是近十余年来的重要进展。其主要特点是采用了衍生化的方法以提高分析对象的挥发性,并改善色谱分离效果及提高检测灵敏度。但在实际分析中,有些衍生物往往会出现一些难分离的物质对,给分析带来困难。在这种情况下需要采用高效和高选择性的色谱柱,或采用几个色谱系统进行分别测定来解决这个问题。我们试验了二次衍生化的办法,即将同一份样品连续进行两次不同的衍生化反应,两次反应的产物分别在同一根色谱柱上进行分析,然后汇总两次测定数据,得到最后结果,作为
The wide application of gas chromatography in biochemical medicine and clinical applications is an important progress in recent ten years. Its main feature is the use of derivatization methods to improve the volatility of the analysis of objects, and improve the chromatographic separation and improve the detection sensitivity. However, in the actual analysis, some derivatives tend to have some difficult to separate material pairs, which brings difficulties to the analysis. In this case, the need to use efficient and highly selective chromatography, or the use of several chromatographic systems were measured separately to solve this problem. We tested the second derivatization method, that is, the same sample was continuously subjected to two different derivatization reactions. The products of the two reactions were analyzed on the same column, and then the data of the two assays were summarized to obtain the final result. As