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目的:观察黏着斑激酶(FAK)在急性白血病(AL)病人白血病细胞中的表达及临床意义。方法:采用半定量逆转录聚合酶链反应(RT-PCR)方法检测50例AL病人白血病细胞FAK mRNA的表达水平,以10例健康人作为正常对照。收集病人骨髓细胞,用24 h常规培养方法制备染色体,采用R显带技术分析其染色体核型。结果:10名正常人骨髓单个核细胞(MNC)中FAK mRNA阳性表达率为20.0%,50例AL病人白血病细胞FAK mRNA阳性表达率为66.0%,明显高于正常对照组(χ~2=5.486,P<0.05);其中急性淋巴细胞白血病(ALL)和急性非淋巴细胞白血病(ANLL)病人FAK mRNA阳性表达率和表达水平均高于正常对照组(χ~2=4.436,P<0.05;t=7.985、5.595,P<0.05),但ALL与ANLL二者之间相比无显著性差异(χ~2=0.0299,P>0.05;t=2.009,P>0.05)。初诊和复发AL病人白血病细胞FAK mRNA阳性表达率和表达水平均明显升高,二者之间无显著性差异(P=1.000,t=1.010,P>0.05),但均高于正常对照组(χ~2=9.38,P=0.015;t=8.789,t=7.5756,P<0.05)。缓解组病人FAK mRNA阳性表达率和表达水平明显降低,均低于初诊组和复发组(χ~2=10.26,P<0.05;t=4.4359,t=7.822,P<0.05),而与对照组相比差异无统计学意义(P>0.05,t=0.5879,P>0.05)。AL病人FAK mRNA的表达与染色体核型的异常改变具有一定的联系,异常核型检出组的表达率和表达水平明显高于未检出组(χ~2=5.82,t=6.72,P<0.05)。结论:FAK mRNA在白血病细胞中表达较高,且与疾病的转归相关,可能为白血病的靶向治疗提供新的位点
Objective: To observe the expression and clinical significance of focal adhesion kinase (FAK) in leukemia cells of patients with acute leukemia (AL). Methods: The expression of FAK mRNA in leukemia cells of 50 patients with AL was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Ten healthy controls were used as normal controls. The bone marrow cells were collected and the chromosomes were prepared by 24 h culture. The chromosomes were analyzed by R banding. Results: The positive expression rate of FAK mRNA in 10 normal human bone marrow mononuclear cells (MNC) was 20.0%. The positive expression rate of FAK mRNA in 50 AL patients was 66.0%, which was significantly higher than that of the normal control group (χ ~ 2 = 5.486 , P <0.05). The positive expression rate and expression level of FAK mRNA in acute lymphoblastic leukemia (ALL) and acute non-lymphocytic leukemia (ANLL) patients were higher than those in normal control group (χ ~ 2 = 4.436, P <0.05; = 7.985,5.595, P <0.05), but there was no significant difference between ALL and ANLL (χ ~ 2 = 0.0299, P> 0.05; t = 2.009, P> 0.05). The positive rate and expression of FAK mRNA in newly diagnosed and relapsed AL patients were significantly higher than those in control group (P = 1.000, t = 1.010, P> 0.05) χ ~ 2 = 9.38, P = 0.015; t = 8.789, t = 7.5756, P <0.05). The positive rate and expression of FAK mRNA in remission group were significantly lower than those in the newly diagnosed group and relapsed group (χ ~ 2 = 10.26, P <0.05; t = 4.4359, t = 7.822, There was no significant difference between the two groups (P> 0.05, t = 0.5879, P> 0.05). The expression of FAK mRNA in AL patients was correlated with the abnormal changes of chromosome karyotypes. The expression and expression of FAK mRNA in AL patients were significantly higher than those in non-detected patients (χ ~ 2 = 5.82, t = 6.72, P < 0.05). Conclusion: FAK mRNA is highly expressed in leukemia cells and correlates with the prognosis of the disease, which may provide a new site for the targeted therapy of leukemia