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Pseudomonasstutzeri1 31 7可以在不同碳源上生长 ,合成不同共聚单体单元组成的中长链聚羟基脂肪酸酯 ( mcl PHAs) .本文在已知的代谢途径的基础上 ,提出了关于 mcl PHAs组成的数学模型 ,结果表明 ,mclPHAs的共聚单体单元组成主要取决于前体生成途径 ,以及该途径和聚合途径酶对不同碳原子数前体的选择性 .实验结果与文献报道结果均验证了该模型 .代谢控制分析的结果表明 ,碳数为 8～ 1 0的前体的聚合效率最高 .通过调制前体生成途径 ,可以有效调节 PHA的单体单元组成
Pseudomonasstutzeri1 31 7 can grow on different carbon sources to synthesize long and medium chain polyhydroxyalkanoates (mcl PHAs) with different comonomer units.In this paper, based on the known metabolic pathways, The results showed that the monomer units of mclPHAs mainly depend on the precursor generation pathway and the selectivity of the pathway and polymerization pathway enzymes to different carbon atom precursors.The experimental results and the reported results verify the model The result of metabolic control analysis showed that the polymerization efficiency of the precursors with carbon number of 8 ~ 10 was the highest, and the monomer unit composition of PHA could be effectively regulated by modulating the precursor production pathway