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目的了解2015年河南省开封市不同来源标本分离的金黄色葡萄球菌肠毒素分布情况及其耐药性。方法依据GB4789.10-2010采用酶联免疫吸附反应(ELISA)方法测定金黄色葡萄球菌肠毒素(SEA-SEE),采用微量肉汤稀释法对检出的致病菌进行抗生素耐药性研究。结果 22株金黄色葡萄球菌中有16株肠毒素阳性,产肠毒素阳性率为72.73%。产1种肠毒素和同时产2种肠毒素的菌株分别是4、12株,在产毒株中分别占25.00%和75.00%。耐药性分析显示22株金黄色葡萄球菌对妥布霉素、氨苄青霉素、青霉素、克林霉素、红霉素、四环素6种抗生素出现高度耐药。结论本次检测的22株金黄色葡萄球菌产肠毒素的菌株占16株,产毒菌株的比率较高,且出现较为严重的耐药情况。
Objective To investigate the distribution and drug resistance of Staphylococcus aureus enterotoxin isolated from different samples in Kaifeng City of Henan Province in 2015. Methods Staphylococcus aureus enterotoxin (SEA-SEE) was determined by enzyme-linked immunosorbent assay (ELISA) according to GB4789.10-2010. The antibiotic resistance of pathogens detected by micro broth dilution method was studied. Results Of the 22 Staphylococcus aureus, 16 strains were positive for enterotoxigenicity and 72.73% for enterotoxigenicity. The strains producing 1 enterotoxin and 2 kinds of enterotoxins simultaneously were 4 and 12 strains, accounting for 25.00% and 75.00% of the strains producing toxins, respectively. Drug resistance analysis showed that Staphylococcus aureus 22 strains of tobramycin, ampicillin, penicillin, clindamycin, erythromycin, tetracycline six kinds of antibiotics appear highly resistant. Conclusion Twenty-two strains of Staphylococcus aureus producing enterotoxigenic strains accounted for 16 strains. The ratio of toxigenic strains was high and there was a more serious resistance.