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目的:通过检测AM患者在位及异位内膜的人类白细胞抗原E(HLA-E)的表达,探讨HLA-E在AM发病机制中的作用。方法:选取2008年12月~2009年10月因AM行子宫切除的标本62份作为病例组,同期因子宫肌瘤行子宫切除的标本20份作为对照。采用免疫组化SP法,检测两组中HLA-E的表达情况,比较检测的结果。结果:HLA-E在对照组内膜组织中低表达,在病例组在位内膜及异位内膜中表达的程度较对照组明显增强,差异有统计学意义(P<0.05),病例组在位内膜组织与异位病灶两者之间比较差异无统计学意义(P>0.05)。细化分组,比较病例组增殖期组与分泌期组HLA-E的表达差异无统计学意义(P>0.05);比较病例组浅层组与深层组HLA-E的表达差异无统计学意义(P>0.05)。结论:①高表达的HLA-E可能参与子宫腺肌病的发病机制;②HLA-E分子表达的强度与月经周期的不同时期及病灶浸润肌层的深度无关;③改善患者免疫状态,为临床治疗子宫腺肌病提供新思路。
Objective: To investigate the role of HLA-E in the pathogenesis of AM by detecting the expression of human leukocyte antigen E (HLA-E) in eutopic and ectopic endometrium of AM patients. Methods: From December 2008 to October 2009, 62 cases with AM hysterectomy were selected as the case group. Twenty cases of hysterectomy specimens for uterine fibroids were selected as control group. Immunohistochemical SP method was used to detect the expression of HLA-E in both groups, and the results were compared. Results: The expression of HLA-E in the control group was lower than that in the control group (P <0.05). The expression of HLA-E in the endometrium and ectopic endometrium of the control group was significantly higher than that of the control group (P <0.05) There was no significant difference between eutopic endometrium and ectopic lesion (P> 0.05). There was no significant difference in the expression of HLA-E between the proliferative phase group and the secretory phase in the comparative group (P> 0.05). There was no significant difference in HLA-E expression between the superficial group and the deep group P> 0.05). Conclusion: ①High expression of HLA-E may participate in the pathogenesis of adenomyosis; ②HLA-E molecule expression intensity and menstrual cycle at different stages and the depth of invasion of myometrial invasion; ③ improve the immune status of patients for clinical treatment Adenomyosis provides new ideas.