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作者研究了非特异性免疫刺激剂卡加苗对小鼠实验感染皮肤利什曼病的影响。以6～8周龄的雌性小鼠分成两组,一组静脉注射1×10~7活的卡加苗,经3周后再与对照组同时在左后脚垫接种5×10~4的热带利什曼原虫。接种后,每周用厚度测量器测量脚垫1次,以比较两组小鼠的左、右后脚垫的肿胀程度。在接种原虫12周后,两组内各有2鼠死亡,取左腘窝淋巴结及肝脾组织分别制成匀浆并通过60孔的铜筛过滤后置NNN基内培养,检查有无鞭毛体,借以了解内脏感染的情况。
The authors investigated the effect of non-specific immunostimulatory agent calgary seed on cutaneous leishmaniasis in mice. Six-to eight-week-old female mice were divided into two groups. One group received 1 × 10 ~ 7 live Calgari, and after 3 weeks, the mice were inoculated with 5 × 10 ~ 4 tropical in the left posterior mat Leishmania. After inoculation, the pads were measured weekly with a thickness gauge to compare the degree of swelling of the left and right rear foot pads of the two groups of mice. After 12 weeks of inoculation of protozoa, two rats died in each group. The left popliteal lymph nodes and liver and spleen tissues were homogenized and filtered through a 60-well copper sieve and set in NNN-based medium to examine whether there were any amastigotes , To understand the situation of visceral infection.