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本文报道两组抗霉菌药,咪唑类(咪唑-哌嗪和miconazole)和多烯(二性霉素乙)在体外对氯喹敏感株(洪都拉斯I/CDC)和抗氯喹株(印度支那I/CDC)恶性疟原虫的作用。在方法上,作者对Trager等的48小时法作了改进,并采用多井组织培养板测定。培养液RPMI 1640内含0.2%碳酸氢钠,25mM HEPES缓冲剂及10%人血清。每井加入9%用贮藏了21～28天的O型红细胞制备的阳性红细胞悬液0.5ml,置37℃孵育室内培养24小时,然后与含有药物的培养液接触,隔24小时换液一次。接触96小时后换成不含药的培养液,再培养48～96小时。于
In this paper, two groups of antifungal agents, imidazoles (imidazole-piperazine and miconazole) and polyene (amphotericin B) were reported in vitro against chloroquine sensitive strains (Honduras I / CDC) and chloroquine resistant strains ) Plasmodium falciparum role. Methodologically, the authors made improvements to the 48-hour method of Trager et al., Using multi-well tissue culture plates. Medium RPMI 1640 contained 0.2% sodium bicarbonate, 25 mM HEPES buffer and 10% human serum. Each well was supplemented with 0.5 ml of a positive erythrocyte suspension prepared by storing 9% O-type erythrocytes stored for 21 to 28 days and incubating indoors at 37 ° C for 24 hours and then contacting with the drug-containing medium and changing liquid every 24 hours. 96 hours after contact with the drug-free medium, and then cultured for 48 to 96 hours. in