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目的观察普萘洛尔对慢性轻度不可预知性应激(CUMS)模型大鼠急性应激前后行为、血清皮质酮水平、海马DG区和CA3区形态学的影响。方法随机将健康成年Wistar雄性大鼠30只分为空白对照组、CUMS组、普萘洛尔处理组各10只。空白对照组大鼠不施加任何处理因素,其他组大鼠经过21 d CUMS暴露制作模型,普萘洛尔组大鼠于每次应激前0.5 h给予普萘洛尔混悬液灌胃。造模结束后,于第22天从3组大鼠中各随机抽取半数大鼠给予一定强度电击,应激结束后2 h取血,采用放射免疫法检测大鼠血清皮质醇的含量换算成皮质酮含量,脑区石蜡切片行HE染色。结果CUMS组大鼠呈现明显的主动活动和探索行为能力下降,海马DG区和CA3区形态学病理改变严重。与对照组比较,普萘洛尔组大鼠急性应激前皮质酮水平明显升高,急性应激后大鼠皮质酮水平显著降低,不能抑制海马DG区和CA3区病理改变,且有加重趋势。结论普萘洛尔可缓解应激导致的焦虑情绪,并降低个体遭遇急性应激时皮质酮的调动。
Objective To investigate the effects of propranolol on acute and post-acute stress, serum corticosterone, hippocampal DG and CA3 morphology in chronic mild unpredictable stress (CUMS) rats. Methods Thirty healthy adult Wistar male rats were randomly divided into blank control group, CUMS group and propranolol group. Rats in the blank control group were not given any treatment factors. Rats in the other groups were exposed to CUMS for 21 days to prepare the model. Propranolol suspension was given orally to the propranolol group 0.5 h before each stress. At the end of modeling, half of rats randomly selected from the three groups on the 22nd day were given a certain intensity of electric shock, 2 hours after the end of the challenge, blood was taken and the content of serum cortisol was measured by radioimmunoassay Ketone content, brain sections paraffin sections HE staining. Results The rats in CUMS group showed obvious active activities and exploreability, and the pathological changes of DG and CA3 in hippocampus were serious. Compared with the control group, the levels of corticosterone before acute stress in propranolol group were significantly increased, while the corticosterone level in rats after acute stress was significantly lower than that in the control group, and could not inhibit the pathological changes of DG and CA3 in hippocampus, . Conclusion Propranolol can relieve the anxiety caused by stress and reduce the individual corticosterone migration in acute stress.