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目的对四川省人民医院肝脓肿患者血培养分离肺炎克雷伯杆菌铁系统基因携带情况进行分析。方法收集2015年1月至12月四川省人民医院检验科保存的10株来源于肝脓肿患者血培养分离的肺炎克雷伯杆菌,提取细菌基因组,通过PCR法测定肺炎克雷伯杆菌iroB、iroC、iroD基因情况,并利用Graphpad Prsim 5.0软件对结果进行分析。结果 10株血培养来源肺炎克雷伯杆菌株中,iroB、iroC、iroD基因阳性的菌株分别有10株、9株和9株,其中iroB/C/D基因均阳性的菌株为9株。结论肝脓肿患者肺炎克雷伯杆菌铁系统基因iroB/C/D阳性率高,同时携带iroB/C/D基因可能为该菌株感染引起肝脓肿的重要因素。
Objective To analyze the gene delivery of Klebsiella pneumoniae isolated from blood in patients with liver abscess in People’s Hospital of Sichuan Province. Methods A total of 10 strains of Klebsiella pneumoniae isolated from the blood cultures of patients with liver abscess were collected from the Department of Laboratory Medicine, Sichuan Provincial People’s Hospital from January to December 2015, and the bacterial genome was extracted. The Klebsiella pneumoniae iroB, iroC , IroD gene status, and the results were analyzed using Graphpad Prsim 5.0 software. Results 10 strains, 9 strains and 9 strains were positive for iroB, iroC and iroD genes in Klebsiella pneumoniae isolates from 10 strains of blood cultures, respectively. Among them, 9 strains were positive for iroB / C / D gene. Conclusions The positive rate of iroB / C / D gene of K. pneumoniae in patients with liver abscess is high. Simultaneously carrying iroB / C / D gene may be an important factor of hepatic abscess.