如果用萋耳尼耳森氏法未檢出結核桿菌時,則可用分離浮游法,此法的檢出陽性率要高得多,將1—3晝夜間吐的痰積存起來,把它注入細頸瓶中,再注入和痰量相等的0.5％苛性鈉溶液,此溶液是由較大濃度的溶液(例如10％新配成的注入後,搖盪3—5分鐘,再放在水浴上加溫55—56°至30—60分鐘, If the Mycobacterium tuberculosis test was not detected by the method using the ear-Nearson’s method, the method of isolation and floatation could be used. The positive rate of this method was much higher, and 1-3% of the sputum accumulated at night was accumulated and injected into the tube. The flask is filled with 0.5% caustic soda solution equal to the amount of helium. This solution is made up of a large concentration of solution (for example, 10% of new solution is injected, shaken for 3 to 5 minutes, and then placed on a water bath to warm it. 55-56° to 30-60 minutes,