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目的:建立RP-HPLC法同时测定芫花药材中芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素和芫花素含量。方法:采用Kromasil C_(18)柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.05%磷酸水溶液梯度洗脱,流速1.0 mL·min~(-1);检测波长为338 nm,柱温40℃。结果:在上述条件下,芫花素-5-O-β-D-茜黄樱草糖苷、芫花素-5-O-β-D-葡萄糖苷、木犀草素、椴苷、芹菜素、羟基芫花素和芫花素的质量浓度分别在1.21～12.1μg·mL~(-1)(r=0.9995),0.797～7.97μg·mL~(-1)(r=0.9997),0.320～3.20μg·mL~(-1)(r=0.9993),1.01～10.1μg·mL~(-1)(r=0.9997),2.02～20.2μg·mL~(-1)(r=0.9999),1.13～11.3μg·mL~(-1)(r=0.9996),1.92～19.2μg·mL~(-1)(r=0.9999)范围内与色谱峰面积呈良好的线性关系,低、中、高浓度的平均加样回收率(n=3)均在96.0%～100.8%,RSD均小于2.8%。结论:该分析方法简便、快速、准确,重现性好,为更好地控制芫花药材的质量提供方法。
OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of 5-O-β-D-cinnamaldehyde glycoside, Daphne geraniol-5-O-β-D-glucoside, luteolin, Tilia glycosides, apigenin, hydroxy genkwanin and genkwanin content. METHODS: Kromasil C 18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of a gradient of methanol-0.05% phosphoric acid in water with a flow rate of 1.0 mL · min -1. The detection wavelength was 338 nm. Temperature 40 ℃. Results: Under the above conditions, genkwanin-5-O-β-D-safrole glycoside, genkwanin-5-O-β-D-glucoside, luteolin, The concentrations of hydroxy genkwanin and genkwanin were in the range of 1.21 ~ 12.1 μg · mL -1 (r = 0.9995), 0.797 ~ 7.97 μg · mL -1 (r = 0.9997), 0.320 ~ 3.20 (r = 0.9999), 1.01 ~ 10.1μg · mL -1 (r = 0.9997), 2.02 ~ 20.2μg · mL -1 (r = 0.9999), 1.13 ~ (R = 0.9996), 1.92 ~ 19.2μg · mL ~ (-1) (r = 0.9999) and the peak area of the chromatogram showed a good linear relationship with the concentration of low, middle and high concentration The average recoveries (n = 3) ranged from 96.0% to 100.8% with RSDs less than 2.8%. Conclusion: The method is simple, rapid, accurate and reproducible, and provides a method for better controlling the quality of Daphne genkwa.