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目的探讨３Ｈ－ＴｄＲ参入ＤＮＡ技术在组织培养中的合理应用。方法测定１６周龄ＳＨＲ和ＷＫＹ大鼠培养的胸主动脉血管平滑肌细胞（ＡＭＳＣ）的自然增长曲线以及接种后６０ｈ期间，每隔４～６ｈ３Ｈ－ＴｄＲ参入的变化。结果ＳＨＲ大鼠ＡＳＭＣ分裂增殖能力比ＷＫＹ强，在１５％ＦＢＳ－１６４０培养基作用下，１～６０ｈ期间，ＳＨＲＡＳＭＣ３Ｈ－ＴｄＲ高峰参入率在２０～２４、５０～５５ｈ之间，ＷＫＹ的高峰参入率在４５～５０ｈ之间。结论要使３Ｈ－ＴｄＲ参入能真实反映培养细胞的ＤＮＡ合成和增殖水平，需根据特定细胞株在某种特定药物干预下的细胞周期，选择适当的３Ｈ－ＴｄＲ标记期，使实验设计更合理。
Objective To investigate the rational use of 3H-TdR incorporation in tissue culture. Methods The natural growth curves of thoracic aorta vascular smooth muscle cells (AMSCs) from 16-week-old SHR and WKY rats were measured and the changes of incorporation of 3H-TdR every 4 to 6 hours were observed 60h after inoculation. Results The SHR rat ASMC had stronger division and proliferation ability than WKY. The SHRASMC3H-TdR peak participation rate was between 20 and 24 and 50-55 h in the range of 1 ~ 60 h under the action of 15% FBS-1640 medium. The peak participation rate of WKY Between 45 ~ 50h. Conclusion 3H-TdR incorporation can truly reflect the level of DNA synthesis and proliferation in cultured cells. The appropriate 3H-TdR labeling period should be chosen according to the cell cycle of a specific cell line under certain drug intervention, so that the experimental design is more reasonable.